Liquid chromatography-electrochemical detection for studying the effects of tetrahydrobiopterin on monoamine neurotransmitters in rat striatum
نویسندگان
چکیده
Tetrahydrobiopterin (BH4) is an essential co-factor in the biosynthesis of monoamine neurotransmitters. A nano-Pt/Pd modified electrode as the electrochemical detector (ED) for high-performance liquid chromatography (HPLC) coupled with microdialysis sampling, is used to explore the effect of administration of BH4 on the monoamine neurotransmitters in the rat striatum. The researches demonstrate that the contents of dopamine (DA), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) increase significantly with the administration of BH4. The pharmaceutical kinetics is carried out to research into the time course of BH4 effect on the concentration of monoamine neurotransmitters in rat striatum, which provides reliable data for pathology and pharmacology research on neuroscience.
منابع مشابه
In Vivo Determination of Tetrahydrobiop- terin and Monoamine Neurotransmitters in Rat Brain by Liquid Chromatography with a Nano Crystalline Mn-Doped Lead Dioxide Film Modified Electrode
The fabrication and application of a novel electrochemical detector (ED) with nano crystalline Mn-doped lead dioxide film chemically modified electrode (CME) for liquid chromatography (LC) were described. The Mn-doped PbO2 film was characterized by scanning tunnel microscope. The electrochemical behaviors of tetrahydrobiopterin, monoamine neurotransmitters and their metabolites at the CME were ...
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